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Retinal slices ( ~ 100 µm thick) were anchored in the recording chamber between Vaseline strips and covered with standard Ringer’s solution (120 mM NaCl, 2 mM KCl, 3 mM CaCl 2 , 1 mM MgCl 2 , 3 mM D-glucose, and 4 mM HEPES, adjusted to pH 7.4-7.5 with NaOH)

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A protective agent against cellular damage